The following instrumentation is available in the PPC Facility on a first-come, first-served basis to trained users. Please schedule an appropriate time block using the Skedda Calendar: https://ouppc.skedda.com/booking
Or contact the director for further information.
The PPC Facility has three separate FPLC units for high resolution protein separations and purifications using custom protocols.
We currently have four separate incubator/shakers available for use with general cellular growth and protein expression.
The shakers utilize a common universal orbital platform and identical flask clamps. As such, use is limited to available space and clamp types on any given date.
We provide a high pressure homogenizer unit for bacterial and yeast cell lysis. The unit can reach pressures in excess of 30,000 psi, making it appropriate for all cell types.
We currently use a fixed angle, large format floor centrifuge for all necessary separation steps during purification.
A large, Class II biologucal safety cabinet is utilized for cell culture procedures.
A CO2 incubator is available in the PPC Facility.
The Facility has an LED inverted microscope available for visualization purposes.
The Facility provides customers with a full-featured imaging unit with the ability to visualize samples using white light, UV light, chemiluminescence, and fluorescence (available Epi-Blue: 460-490 nm excitation and Epi-red: 625-650 nm excitation).
The Facility recently acquired a pUNk dynamic light scattering system for assessing a protein's hydrodynamic size, size distribution, and aggregate population.
The Facility recently acquired a NanoDrop spectrophotometer which can quantify protein and nucleic acid samples and obtain full spectral data using only 1-2 ul of a sample. A cuvette position is also available.
The Facility has a Synergy water purification system available for supplying users with quality ultrapure water.
For insect cell cultures, we utilize a separate refrigerated incubator containing cell-spin containers.
SLSRC 2590 | Norman, OK 73069 | 405-325-1529